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蛋白纯化
  • DP101-01.JPG
DP101-01.JPG

ProteinIso® Ni-NTA Resin


产品介绍

ProteinIso®  Ni-NTA Resin是螯合金属Ni2+而形成的一种亲和层析介质。NTA能够通过四个位点牢固的螯合Ni2+从而减少纯化过程中Ni2+泄露到蛋白样品中。本产品对His标签蛋白具有特异吸附能力,从而使His标签蛋白结合在Ni-NTA纯化介质上,未结合的蛋白被洗涤下去,结合在介质上的蛋白经过一定浓度的咪唑或低pH缓冲液温和洗脱下来,从而得到高纯度的目的蛋白。本产品具有吸附容量大、选择性好,通透性强,易于再生的优点。它可用于在非变性或变性条件下纯化任何表达系统表达的His标签重组蛋白。  

References

Molecular characterization of LvAV in response to white spot syndrome virus infection in the Pacific white shrimp (Litopenaeus vannamei)[J]. Developmental & Comparative Immunology, 2015, 51(1):48-55.

Cao H, Yin X, Cao Y, et al. FCHSD1 and FCHSD2 Are Expressed in Hair Cell Stereocilia and Cuticular Plate and Regulate Actin Polymerization In Vitro[J]. PLOS ONE, 2013, 8(2):e56516.

Yang X Q, Wang W, Tan X L, et al. Comparative Analysis of Recombinant Cytochrome P450 CYP9A61 from Cydia pomonella Expressed in Escherichia coli and Pichia pastoris[J]. Journal of Agricultural and Food Chemistry, 2017, 65(11):2337-2344.

Cheng S , Li C , Wang Y , et al. Characterization and expression analysis of a thioredoxin-like protein gene in the sea cucumber Apostichopus japonicus[J]. Fish & Shellfish Immunology, 2016, 58:165-173.

Liu Y, Hou F, Wang X, et al. Recombinant expression and characterization of a serine protease inhibitor (Lvserpin7) from the Pacific white shrimp, Litopenaeus vannamei[J].2015 Feb;42(2):256-63.

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